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Moreover, we used a panel of antibodies including a sMyBP C antibody that recognizes constitutively expressed epitopes in the C5 domain ( pan sMyBP C), and four phospho specific antibodies recognizing all known phosphorylation sites ( sMyBP C mSer59P, sMyBP C mSer62P, sMyBP C mThr84P, and sMyBP C mSer204P). It is important to note that due to the presence of multiple sMyBP C variants with similar molecular weights (126 131.5kDa)10, the immunoreactive bands detected in our phosphate affinity blots may correspond to unique variants phosphorylated to differing extents, differentially phosphorylated forms of the same variant, or a combination of both. Consequently, we refrain from denoting those as hyper or hypo phosphorylated species and instead refer to them according to their electrophoretic mobility as low, intermediate, or high.

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